It further conducts double-quantification studies on granular computing and rough set models.
This system provides a double-redundant quantification of the temperature, topology and emissivity distributions of an optically opaque diesel-flame with a temporal and spatial resolution of 10,000 images/s and 16 Pinels/mm2 in this set-up.
Network branch number counting was performed by a single investigator who was not the person who captured the images, thus ensuring a double-blind quantification method.
DNA concentration was quantified using the PicoGreen double strand DNA Quantification Kit (Molecular Probes Inc., USA).
We use SYTO9 dye for double stranded DNA quantification, as it has been shown to have fewer sequence and concentration artifacts (Gudnason et al. 2007).
Double stranded DNA quantification assay (The Quant-iT PicoGreen™ Assay, Invitrogen Carlsbad, CA) was performed per manufacturer's instructions; double stranded DNA extracted from bovine thymus was used to create standards of 1,000, 100, 10, and 1 ng/mL.
cDNA synthesis, cRNA double amplification, labelling, quantification, quality control and fragmentation were performed with an automated system (Caliper Life Sciences NV/SA, Teralfene, Belgium), starting with 10 20 ng total RNA from each sample, all as previously described in detail [ 80, 81].
For images acquired at half the exposure time, the corresponding average intensity over the field of view was doubled for the quantification study.
The method is well validated against the gold standard for energy intake quantification (double-labelled water) and has good reproducibility [ 18– 20].
