Sentence examples for double pulse protocol from inspiring English sources

Using a double pulse protocol (see [25]) mean recovery kinetics at HP = −100 mV (single exponential fits) were significantly faster for V287R than for WT (WT: τrec = 185 ms, V287R: τrec = 30.10 ms, Figure 3.A).

Steady-state inactivation was determined with a double pulse protocol consisting of 500 ms prepulses from the holding potential of −120mV (or −140mV for R1632H only) to voltages between −140 and −20 mV (or −160 and −20mV for R1632H only), followed by a constant test pulse of 20 ms duration to −20 mV at a pulsing frequency of 0.5 Hz.

Recovery from inactivation was determined with a double pulse protocol consisting of 500 ms prepulses to −20 mV, followed by variable recovery intervals at −120 mV, and a constant 20 ms test pulse to −20 mV at a pulsing frequency of 0.25 Hz (or 0.15 Hz for R1632H only).

Time constants (τrec) for recovery from fast inactivation were obtained by a single exponential fit to the time course of recovery in a double pulse protocol in which inactivation is induced by a 40 ms prepulse to −20 mV, followed by a variable duration recovery at different conditioning potentials, and then tested by a depolarization to −20 mV.

A double pulse protocol was used to access the time course of recovery from inactivation.

We next investigated the characteristics of INa and ICa using a double pulse protocol (see Materials and methods and Fig. 3A).

To measure the recovery of ASICs from desensitization, we evoked ASIC currents in nucleated patches from NG2 cells by applying a double-pulse protocol (Fig. 4C).

Indeed, the V1/2 obtained with the double-pulse protocol was −20.3±4.4 mV, which is much more positive than the V1/2 of current obtained using this same protocol in WT hERG channels (about −80 mV) [33] [36].

Effects on NaV steady-state inactivation were tested using a double-pulse protocol.

The above findings are supported by TMS studies using the double-pulse protocol for assessing inter-hemispheric inhibition.

The currents evoked by the second pulse of this double-pulse protocol were normalized to the currents produced by the first pulse and plotted against the duration of the inter-pulse interval.