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The results suggest that the strategy of combining the 2A peptide and double promoters efficiently mediates the co-expression of the four fluorescent proteins in pigs and is hence a promising methodology to generate multi-transgenic pigs by a single nuclear transfer.

Within 200 bp distance, only 25 double promoters (0.03% of 863 total gene pairs) were identified.

The effect of double promoters was examined by using double 400 or 700bp BnMYB80 5'UTR sequences to drive the BnMYB80-32R2 constructsonstructs (P Bn400x2 :Bn80-32R2 and P Bn700x2 :Bn700x2R2).

Between the genomic region of 2 genes, the proportion of STAT1 binding sites of double promoters was higher than other 3 promoter categories, which indicates that these STAT1 binding sites are important in recruiting RPol II for both genes.

The regulatory regions located in the bidirectional region were divided into 4 categories, double promoters which included 2 individual promoters, left promoters that only cover the TSS of gene in negative strand, right promoters that only cover the TSS of gene in right stand, and centre promoters that cover 2 TSSs. Figure 4A shows the RPol II binding pattern of four types of promoters.

We have reported on a bioinformatics strategy to look at regulatory regions in bidirectional promoter regions, based upon ChIP-seq-derived genome-wide binding patterns of transcription factors and RNA polymerase II; there are 249 double promoters, 76 right promoters, 86 left promoters and 932 centre promoters.

Similar(54)

In this study, we described a strategy to improve the expression efficiency using synthetic double promoter.

The synergistic effect in the double promoter was observed only when σB-promoter was located upstream to σA-promoter but independent to its orientation.

Although deletion variants continued to arise by other means, they arose at a much lower rate in comparison to the double promoter viruses [30].

A vector to direct recombination of two gene expression cassettes under the control of early viral promoters into MVA deletion site III was constructed by replacing the synthetic double promoter in plasmid pG06 [69] with bidirectional early (modified H5) viral promoters.

In double promoter and centre promoter categories, > 60% paired genes were expressed.

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