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There are two peak periods of rainfall corresponding to the double passage of the ITCZ.
The light's double passage through the pigment creates an intense yellow.
In brief, 1×106 cells/test were lysed in 1 ml of ice-cold lysis buffer (1% Triton X-100, 150 mM NaCl, 1 mM NaF, 1 mM EGTA, 1 mM EDTA, 1 mM phenylmethylsulfonyl fluoride (PMSF), 2 µg/ml aprotinin, 2 µg/ml leupeptin, 1 mM sodium orthovanadate and 50 mM Tris-base, pH 7.4) by double passage through a 26½-gauge needle.
Human cervical cells lines were magnetically labeled and separated by double passage with 1 μl CD133 (CD133/1 clone, Miltenyi Biotec) and 1 μl CD34 microbeads (QBEND/10 clone, Miltenyi Biotec) per 1 × 106 cells, utilizing the Miltenyi Biotec CD34 and CD133 cell isolation kit.
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It examines the effect of the operating variables: air and water flow and double passages through Honeycomb II, on the MTBE removal.
In this work we reported that by modifying the propagation regimen and growth conditions (6T15 protocol) MEF slowed down without reaching a complete cell proliferation block and restarted proliferation after about eight population cell doubling (passage 3).
Fetal bovine serum favors fibroblastic morphology with enhanced proliferation rate, population doubling, passage number and triggers cell differentiation (as vimentin staining showed), suggesting serum factors essential for the equine airway fibroblasts are available in the fetal bovine serum, whereas in the horse serum cells there were signs of degeneration or cell granularity.
The mirrors at the far ends of the cells have a high reflectivity in the 1083 nm range but high transmission for the 668 nm fluorescence light, providing a low-loss double-passage of the 1083 nm light through each cell while permitting to monitor the degree of 3He spin polarization with an optical polarimeter (OPN) by polarization analysis of the 668 nm fluorescence light.
Normal human fetal lung fibroblasts (AG06814N, WI-38) at population doubling 15 (passage 12) were obtained from the National Institute of Aging Cell Culture Repository Coriell Medical for Medical Researchh, Camden, NJ).
Cell proliferation was measured as number of population cell doubling per passage (CD= ln(Nf/Ni /ln2) where Nf is the final number of collected cells (day 3) and Ni the initial number of seeded cells (day 0).
For parametric data, two-tailed paired t-tests were performed for comparisons between two different media conditions, while analyses of variance (ANOVA) followed by Bonferroni correction were performed for comparisons of more than two groups (population doubling per passage, passage time, ALP content and calcium deposition).
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