Sentence examples for double nick from inspiring English sources

This has prompted development of the "double nick" technique that requires the coordinated activity of a pair of mutated Cas9 proteins targeted to neighbouring sequences, to improve sequence specificity (Mali et al., 2013a; Ran et al., 2013).

Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity.

Ran, F. A. et al. Double nicking by RNA-guided CRISPR/Cas9 for enhanced genome editing specificity.

Ran, F.A. et al. Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity.

Ran, F. A. et al. Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity.

To achieve homozygous knock-in clones with long DNA templates, a "double nicking" strategy might be employed with paired offset guide RNAs to significantly increase the homozygous targeting efficiency (Ran et al., 2013).

When inducing double nick-mediated genome modification, the optimal range of the offset length is around 0 10 bp [ 23], whereas FokI-dCas9 requires 13- to 18-bp offsets for highly-efficient targeted mutagenesis [ 14].

For example, the paired-gRNA guided double nicking Cas9 system could be applied to extend the length of recognition sequence and reduce potential off-targets [ 19].

Interestingly, the requisite for a 5′ cut orientation is consistent with previously reported application of double nicking systems in mammalian cells.

Recent modification of the CRISPR system to use double nicking of single strands has dramatically decreased off target cleavage events (Ran et al. 2013a).

Although a double nicking strategy is newly developed to enhance the CRISPR cleavage specificity [ 16], it renders limited help in this situation as doubling the DNA recognition length does not necessarily confer more selectivity between two highly homologous genes.