We also made a double mutation N316AF320A, in which both Asn316 and Phe320 were mutated to Ala.
We mutated each target site alone and also made a double mutation changing both sites.
The double mutation of both residues further compromises cation selectivity.
Wild-type, single mutation, and double mutation were observed in 29, 20, and 13 parasites, respectively.
The covariance and PCA analysis elucidated that the double mutation affects the collective motion of residues in phase space.
However, H2O2 enhanced expression of the RAGE harboring C259S/C310S double mutation (DM-RAGE) was observed in the endoplasmic reticulum.
Furthermore, the flexibility and compactness analyses indicate that the double mutation influence interaction of PZA with the protein.
Lastly double mutation experiments reveal an interaction between D2R and D3R with the former prevailing on the latter.
Then, the double mutation constructs K1117/1127R and K1389/1413R were constructed.
They were similarly inconsistent with the hypothesis that the double mutation blocked pollination.
Comparably, in H. sapiens AChE the same double mutation increases Ki for the inhibitor 35-fold.
