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Single-labeled and double-labeled surface as well as the mean distance between double labels were measured at uncoulored bone sclices of the same region used for histomorphometry by fluorescence microscopy (Axioplan, Carl Zeiss, Jena, Germany).
Larger meals and portions of fries have a triple-prize label, while the medium portions have double labels, incentivising customers to "go large" and increase their chances of a win.
MAR was calculated as mean distance between the double labels divided by the number of the days between the calcein injections.
Qualitative analysis of bone histology also revealed an increase in unmineralized osteoid surfaces (Fig. 6E) and diffuse calcein labeling of bone compared to the distinct double labels in wild-type mice (Fig. 6F), indicative of impaired mineralization.
The distance between the two labels was measured in 10 randomly chosen fields per slide, and the mineral apposition rate (µm/day) was determined by dividing the mean distance between the double labels by the interlabel time (4 days).
If so, having double labels would be more convincing.
Similar(43)
We further observed nestin and MAP-2 expression by Western blot and IF double labeling.
Thawed cryosections were double labeled for NP (10 nm) and PB2 (15 nm).
DNA replication protocols provided are for double labeling DNA from an asynchronous population of cells.
BrdU single and double labeling with neuronal markers were used to analyze cell proliferation and differentiation.
Double label experiments provided evidence that α4 and β2 subunits co-localize on small RGCs.
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