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The tetracycline double labelling of bone was used to calculate kinetic data on bone turnover.
These results were further confirmed by double labelling experiments.
These countings were performed as the other double labelling countings.
For double labelling, we applied a second primary antibody for 1 hour.
Satellite cells were enumerated via double labelling with an anti-Pax7 antibody and DAPI.
In the double labelling experiments the two types of secondary antibodies were applied together.
Similar(23)
Double-labelling immunofluorescence was performed as previously described [25].
Double-labelling using fluorescent stains is a well-established method for visualising different anatomical features in brain tissue (e.g., [46]).
Double-labelling with TH indicated that many DA neurons are netrin-1 immunoreactive at both ages (Fig. 5).
Scoring of the double-labelling was carried out from photomicrographs.
Compared to double-labelling these results for quantitative single-labelling are not as good and there are also other disadvantages compared to double-labelling (please see Discussion).
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