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Figure 3 Double labeling FISH using QD525 and QD585 oligonucleotide probes.

DNA replication protocols provided are for double labeling DNA from an asynchronous population of cells.

BrdU single and double labeling with neuronal markers were used to analyze cell proliferation and differentiation.

We tested this prediction by double labeling.

Cells were rotated in orthogonal planes to verify double labeling.

Double labeling was performed by simultaneous incubation with the respective primary antibodies.

Besides, this method was simple in design, avoiding double-labeling of probe.

We therefore performed this double-labeling on PSR rats (n = 4) only.

PER, CLK quantitation: Cells were identified by position, size, and PDF double-labeling when applicable.

Double-labeling immunofluorescence was performed by sequential incubation with the appropriate primary antibodies.

Fig. S4 Double-labeling EdU+ HuC/D.