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Double imaging is needed to create ultraprecise three-dimensional maps of the planet's peaks and valleys, as far north as Alaska and as far south as the tip of South America.
The coupling between the formation of new adhesions and the advance of the lamellipodium/lamellum boundary was evident both in the enhanced phase contrast/YFP-paxillin double imaging mode and in the rhodamine-actin/YFP-paxillin double fluorescence mode (Supporting Movie S8).
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As a solution, the GPU-accelerated full-range FD-OCT has been utilized and real-time B-scan images was demonstrated with effective complex-conjugate suppression and doubled imaging range [ 11, 12].
Noise and error including double tip imaging appeared to cause the change in periodicity.
Consistent with recent reports [25], [33], this was confirmed by double fluorescence imaging of the markers of stress fibers and focal adhesions.
Double florescence imaging with microinjected rhodamine-myosin II and YFP-paxillin demonstrated a flow of myosin-positive spots to the center of the cell away from the adhesion sites with velocities similar to the velocity of the lamellar flow component (Figure 3A, and Supporting Movie S10).
This two-step procedure allowed us to separately optimize the acquisition rate and the alignment precision for single and double channel imaging respectively.
PTP status was assessed by double channel imaging of NAD(P H autofluorescence and mitochondrial electrical membrane potential (i.e. tetramethylrhodamine methyl ester (TMRM) fluorescence) as recently described, whereas Ca2+-induced PTP opening was triggered off by exposing INS-1 cells to the Ca2+ ionophore A23187.
Proton electron double-resonance imaging (PEDRI) offers rapid image data collection and high resolution for spatial distribution of paramagnetic probes.
Benefited from double-shot imaging system, dynamic scene could also be handled.
This paper describes the design, construction and use of a field-cycled proton electron double-resonance imaging (FC-PEDRI) system for the detection and imaging of free radicals.
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