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For triple labelings (NPY, 5-HT1A and 5-HT2C mRNA), we applied double FISH using HS-TSA-FISH for 5-HT2C and LS-TSA-FISH for NPY mRNA detection, respectively, followed by CISH for 5-HT1A mRNA detection, DAPI-staining and mounting.
For NPY/5-HT2C double FISH, first HS-TSA-FISH was applied to detect 5-HT2C mRNA.
Double FISH and immunohistochemistry coupled to FISH were performed as described in (Andrikou et al., 2013).
Fluorescent in situ hybridization (FISH) using LNA probes and double FISH to detect miR-9/Hes1 expression in the c17.2 cells were performed as previously described (Dajas-Bailador et al., 2012) with some modifications.
The strongest hybridization signals were obtained in double FISH with a mix of mRNA and U2 snRNA, so an attempt was made to use double FISH for U2 snRNA as a marker of CB and mRNA of distinct genes.
"Double Happiness and Double Fish [another Chinese brand] provided the equipment for Chinese teams to dominate the ping pong world for nearly 60 years," says Deng Yaping, who won six world championships and four Olympic gold medals with the company's blades.
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(Several double-FISH tests would quickly confirm this).
Double-FISH techniques were performed according to [ 10].
(D ) Double-FISH with smedwi-1 and Smed-FoxA at different times after pharynx removal.
Double-FISH treatment with (GATA n and 18S rRNA gene probes showed a non co-localized pattern.
The double-FISH treatments with 5S-18S and 5S-GATA probes had been performed in previous work [ 10].
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