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For microarray experiments, total RNA was extracted by a double extraction protocol.
Fe(III) (50 200 µg) can be extracted from aqueous solution with 1% solution of 8-hydroxyquinoline in chloroform by double extraction when the pH of the aqueous solution is between 2 and 10.
The results of these studies confirmed the superiority of (5AB-8HQ) in double extraction of Mg II) due to the presence of four reactive functional groups including the quinoline OH and N as well as the azo and carboxyl groups compared to (5PA-8HQ) and (8HQ).
The best extraction yield (99.9%) was achieved with the solvent 1,1,2-trichlorotrifluoroethane, an extraction solvent volume/sample volume ratio of 10∶1 and a double extraction.
Articles were abstracted using a standardized data collection template with double extraction on a sample to ensure quality.
The double extraction of the same fiber, with formic acid followed by hydrochloric acid, provided more information than a one-stage procedure.
Similar(51)
Zero, single and double extractions models are evaluated for the conventional CAOW water heated cycle.
The influence of DMF to diesel ratios, extraction times and temperatures, addition of co-solvent, solvent mixtures, double extractions were explored.
In 3/135 (2.2%) cases prospectively tested for diagnostic purposes, all from metastatic CRC, we had to perform double extractions from (a) the whole section and (b) upon macrodissection of the neoplastic site, due to reported discrepancies on KRAS mutation status assessed in different labs.
The residual aqueous phase was subjected to the double extractions with ethyl ether.
The RisN method [29] was preformed with doubled extraction volume.
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