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Mr. Levy said he had omitted the threatened cuts because he had grown more confident of the passage of the sales tax, and he wanted to avoid the double disruption of making deep cuts and then restoring them.
Especially, the strain with combination of OAZ1 and TPO1 double disruption and overexpression of SPE3 not only contained spermidine content of 1.1 mg SPD/g cell, which was 171% higher than that of the control strain, but also exhibited 60%and33%3% shorter lag-phase period than that of the control strain under the medium containing furan derivatives and acetic acid, respectively.
A double disruption of sepF and ftsA completely eliminates Z-ring formation and is lethal.
For example, a double disruption of two of the four pectate lyases (PEL) present in the pea hemibiotroph pathogen Nectria haematococca genome rendered mutants drastically reduced in virulence [ 46], but single gene knockouts did not affect virulence.
To examine the effect of DNA damage we chose a dose of NQO (10 μg/ml) at which we would expect to be able to readily detect an additive effect of the double disruption (see Fig. 4D).
Similarly, the disruption of formate production [ 64, 65] as well as double disruption of formate and ethanol production [ 64] in C. reinhardtii also results in rerouted fermentative pathways, yielding changed metabolite profiles, especially for those metabolites that require NADH as reductant.
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We previously reported that the organic solvent tolerance of strain JA300 significantly improved the double disruptions of marR and proV (Doukyu et al.2012).2012
Double disruptions of the superior suspensory shoulder complex, commonly referred to as 'floating shoulder' injuries, are ipsilateral midshaft clavicular and scapular neck/body fractures with a loss of bony attachment of the glenoid.
Double disruptions of the superior suspensory shoulder complex (SSSC) resulting in ipsilateral midshaft clavicular and scapular body/neck fractures, are commonly referred to as a 'floating shoulder' injury, and result in a loss of bony attachment of the glenoid [1, 2].
The S. pombe strains used in this study were the wild-type FY435 (h+ his7-366 leu1-32 urandΔ18 ade6-M210) and the ctr4Δ ctr5Δ double mutant disruption strain (isogenic to FY435 plus ctr4Δ::ura4+ ctr5Δ::KANr) [22].
However, none of the homokaryotic transformants analyzed showed the double Δprs2,4- Δprs3 disruption.
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