Sentence examples for double dig from inspiring English sources

Sections were permeabilized using 40 µg ml−1 Proteinase K (Novagen, WI, USA) in TE buffer at 37°C for 15 min followed by hybridization with a mixture of two 5' and 3' double DIG labelled LNA probes (5'-CACCATCAGTGAACTTAGGAGCAAC-3' and 5'-CATACTCCAAGATCATCGCCAGCA-3') (250 nM each) for 18 hours at 50°C.

The specificity of the in situ hybridisation was determined by a negative scramble double DIG labeled LNA probe (Exiqon) that did not give any signal (Supplementary Figure 1).

How to Double Dig a Garden.

See wikiHow's article on how to double dig a garden for instructions.

It comprised raised beds that were double dug and boosted by compost.

But many rocks, stones and pebbles, like the ones that keep floating up from the soil when you are double digging.

His insistence on double digging — excavating the soil down two spade heights (about two feet), mixing it with organic conditioners and then mounding it in un-edged beds — makes for a backbreaking project.

The project, run by charity Send a Cow and funded by DfID's Development Awareness Fund, gets farmers from the UK and Africa building African gardens in British farms and demonstrating approaches and techniques such as key-hole gardens, bag gardens, double dug beds, natural pesticides and plant teas.

We performed nonradioactive ISH on paraffin tissue sections with a double DIG-labeled probe according to the manufacturer's instructions (Exiqon, Copenhagen, Denmark).

Whole-mount in situ hybridization using double DIG-labeled LNA oligos (Exiqon) or antisense RNA probes was carried out as previously described (Goljanek-Whysall et al., 2011).

Slides were then pre-hybridised at 50 °C in a hybridisation oven for 30 min followed by hybridisation with 200 n M double DIG-labelled LNA-modified probes for an hour at 50 °C.