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To confirm whether these were induced from 2-oxoacids with reactive carbonyl groups, a test sample was additionally prepared using a double derivatization protocol that preferentially protects carbonyl groups [ 44].
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Evaluations of these data concluded that many, but not all, products derived from "double derivatization" (sequential derivatization with two derivatization groups), generate ions of higher quality than those derived from "single derivatization".
Following two different investigations an extraction and derivatization protocol was chosen.
We have developed a "one-pot" extraction and chemical derivatization protocol using N-methyl-N- tert-butyldimethylsilyl) trifluoroacetamide (MTBSTFA) aN-methyl-N- tert-butyldimethylsilylSample AN-methyl-N- tert-butyldimethylsilylstrumeN-methyl-N- tert-butyldimethylsilyl Laboratory (MSL) missioN-methyl-N- tert-butyldimethylsilyl
The derivatization protocol described by Wu et al. (2013) was used.
Thus, the derivatization protocol of the current study could strikingly be used for simultaneous determination of both β-alanine betaine and TMA in plant, food, and microbial samples.
The derivatization protocol was modified from protocols described previously [ 10, 16, 18].
This was expected as the derivatization protocol favored the extraction of these compounds.
The colloidal gold protein staining patterns that resulted after the derivatization protocol, were essentially the same as the protein patterns observed when the proteins were stained without derivatization.
To investigate the significance of the observed changes in ROS, we measured the effect of p38 MAPK inhibition on the overall oxidation state of cellular proteins using a chemical derivatization protocol.
However, derivatization protocols must also be developed if GC/MS (one of the mostly widely used analytical methods for metabolomics) is involved.
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