Sentence examples for double cross over from inspiring English sources

To study the function of POFUT2 in P. falciparum, isogenic NF54 parasites were generated in which the POFUT2 locus was excised by double cross over homologous recombination (Supplementary Fig. 8A).

Rescreening the colonies for a single crossover on a new plate supplemented with kanamycin showed the double cross over pattern for most of the resulting colonies.

Upon a double cross over event this vector is predicted to disrupt the entire PbPlrx open reading frame (ORF).

The double cross over mutant, KO48-DCO in M.smeg mc2155 (Fig. 8B, lane 5), was used for further experiments.

Integration of the cassette by single or double cross over events was verified by PCR for 3 randomly selected clones for each construction.

Parasites of line 676m1cl1 contain the PbGFP-Luc gene fusion stably integrated as a single copy gene by double cross over recombination into the 230p locus and the reporter gene is under control of the constitutive eef1aα promoter [39].

To test our approach, we randomly selected 10 and then 96 genes for mutagenesis by double cross-over homologous recombination.

Allelic exchange mutants generated by double cross-over homologous recombination were selected by two-day microaerobic incubation on BHI agar plates containing 500 μg/ml kanamycin.

The sspA was then deleted from the S. spiramyceticus F21 genome by double cross-over homologous recombination mediated by temperature-sensitive plasmid pKC1139.

Using a double cross-over study design, subjects were randomized to one of two arms and crossed-over one week later: control arm – no calf exercise while sitting; intervention arm – calf contraction against a pedal resistance while sitting.

The crtYB gene, encoding the bifunctional enzyme phytoene synthase/lycopene cyclase, was inactivated by insertion of a vector by single and double cross-over events, indicating that it is possible to generate specific carotenoid-biosynthetic mutants.