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Peripheral blood samples were processed by a double centrifugation protocol as previously described [30].
That study included 29 canine blood samples collected with EDTA by using a double centrifugation protocol [ 30].
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Maternal plasma was harvested from EDTA-blood by our previously established double-centrifugation protocol [26].
By using sample processing protocol concerning the double centrifugation of blood samples at 1200 g we found out comparable fetal DNA levels as those reported by Lo et al. who separated the plasma using double centrifugation at 3000 g [ 6].
Those protocols included simple and double centrifugation steps for concentrating between 400 X 10 to 1300 X 10 PLT/μL.
Platelet concentration reached in the protocol described here was slightly lower than a semi-automated double centrifugation method evaluated in dogs, which presented a median concentration of 1336 PLT X 10/μL [ 23].
The presence of parasites was determined using either the modified single centrifugation (Malawi) [24] or double centrifugation (Uganda) [25] methods.
Cytosolic, mitochondrial and nuclear protein fractions were obtained by a differential centrifugation protocol.
PBL were isolated according to the Ficoll-Paque density centrifugation protocol.
The recombinant Ad5 vectors were purified by double centrifugation on cesium chloride gradients and subjected to dialysis as described [48].
Swarmer cells were isolated from mixed population cultures using a modified version of the percoll density centrifugation protocol [65].
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