Thus, the majority (62.9%) of these NFI-bound double boundaries did not map to known transcriptional initiation or termination sites (92 out of 148 double boundaries, Table 1).
This indicated that the association of NFI with the double boundaries does not result from fortuitous coincidence.
Whether some of the 92 NFI-bound double boundaries may correspond to as yet unknown imprinted loci remains to be evaluated.
We therefore conclude that most NFI-associated H3K27me3 and H3K36me3 boundaries cannot be simply ascribed to chromatin modifications elicited by mRNA transcription, and that over 90 of these double boundaries can result from NFI binding alone.
From the 1,148 double boundaries that are not localized at known TSS or TES, 92 (8.01%) were bound by NFI, whereas only around 2.4% were found to occur by random coincidence.
This finding was specific of the H3K27me3 and H3K36me3 double boundaries, as a similar analysis of H3K27me3-only boundaries that do not overlap TSS or TES did not reveal an enrichment of NFI as compared to randomly selected genomic sites (1.04% vs. 1.16%, Table 1).
Further large scale genomic studies will be required to assess the intriguing possibility that such double boundaries may be indicative of as yet unknown imprinted loci and whether altered imprinting may contribute to some of the developmental and tissue regeneration abnormalities observed in NFI-C knock out mice [ 30, 50, 55].
Using a two-mode lumped model, we present theory and comprehensive bifurcation analysis of thermally coupled homogeneous heterogeneous combustion of propane and methane in short monolith, fibermat or gauze type reactors with a focus on the dependence of the ignition, extinction, hysteresis, double and boundary limit loci on the various design and operating parameters.
A double periodic boundary condition is used along x (along the latitude line) and y (along the longitude line).
The calculation domain covers 12.8 × 12.8 km2 horizontally with a double periodic boundary condition and 4.0 km vertically.
The present work analyzes the double diffusion boundary layer flow of a viscoelastic nanofluid over a stretching sheet including the nonlinear thermal radiation, mixed convection, and magnetic effects.
