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At least 500 cells per slide and three slides per condition were double-blind counted.
Figure 1(c) shows the statistic differences between the groups after a double blind count of PM thickness in ten arbitrarily chosen fields of optical microscopy.
Comparable results were obtained by manual double-blind counting (results not shown).
The bud scars were counted double blind from confocal z-stack images.
The number of tyrosine hydroxylase-, Chand and NeuN- immunopositive or Nissl-stained cells was stereologically estimated by double-blind cell counting in bright field microscopy using an optical fractionator method (King et al., 2002).
Averages of manual double blind TH+ or fluorescent counts of three wells of culture with the same treatment were collected; counts were performed in triplicate.
Volunteers also counted some of the preps, selected at random, in a double blind manner.
In the immunohistochemistry experiments, all PCNA positive nuclei were counted in five independent fields by two independent observers in a randomized double blind fashion.
Double blind method.
Blinding, as in "double blind," is a defense against bias.
Design: Double blind RCT.
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