The double anchoring of the enzyme to ssDNA can result in (i) an enhancement of hAID pausing on the substrate, which may increase the deamination rate, and/or (ii) a decrease in the rate of product dissociation, which may slow down the deamination process.
(c) The ability of wt hAID to pause and deaminate 5 mC is determined by (1) sufficient stabilization of the pyrimidine ring in the catalytic pocket; (2) double anchoring of the sugar-phosphate ssDNA backbone (by interactions specific to the N51 residue and to the methyl group); and (3) unstable stacking (W84 Y114 G) or alternative stacking (A Y114 G).
The results from these studies show that n-alkyl dicysteinyl peptides (CP 1 4), and an aryl dicysteinyl peptide (CP 5) can serve as effective "double anchors" to accommodate the coordination sites of mercury II) to form predominantly one-to-one Hg peptide) complexes.
Fifty years ago, Victor Gruen designed a fully enclosed, introverted, multitiered, double-anchor-tenant shopping complex with a garden court under a skylight and today virtually every regional shopping center in America is a fully enclosed, introverted, multitiered, double-anchor-tenant complex with a garden court under a skylight.
Fifty years ago, Victor Gruen designed a fully enclosed, introverted, multitiered, double-anchor-tenant shopping complex with a garden court under a skylight — and today virtually every regional shopping center in America is a fully enclosed, introverted, multitiered, double-anchor-tenant complex with a garden court under a skylight.
They found that hetero bi-functional reactive dyes afford great chance for efficient reaction between dye and fiber due to the mixed double-anchors.
Also this filtering representation at multiple scales might be the underlying mechanism to connect similar explanations such as ours with some mid- to high-level explanations for example 'Anchoring theory' [26] and its extensions such as 'Double-Anchoring' [76] and the idea of illumination framework proposed to address brightness induction effects.
The groove will be debrided, and a metal double suture anchor with needles will be placed in the groove.
It was found that among the three coaxial mixers considered in this research work, the mixing time achieved by the double Scaba-anchor coaxial (DSAC) mixer was the lowest.
