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After washing, blotting dots were visualized by Immobilon Western Chemiluminescent HRP substrate (Millipore, Guyancourt, France).
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PLA signals are shown as red dots and nuclei were visualized by DAPI staining (blue).
Distributions of the positive and negative hits (77 GM-EIA OD450/620 values, 85 PCR Cq-s) were visualized by dot histogram (Additional file 1: Figure S1).
The beads were first exposed to the control serum and, after washing, beads binding IgY were visualized by the addition of an anti-IgY secondary antibody conjugated to a red quantum dot (Qdot655).
Liquid droplets were visualized by DIC microscopy.
Nuclei were visualized by DAPI (blue).
Both particles were visualized by TEM.
The membrane reactions were visualized by Perkin Elmer-enhanchemiluminescenceence reagents.
Following secondary antibody incubations, signals were visualized by enhanced chemiluminescence.
Bands were visualized by enhanced chemiluminescence (Pierce Biotechnology).
The nuclei were visualized by DAPI staining (blue fluorescence).
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