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The screen was scanned by Typhoon Trio phosphorimager (GE Healthcare, USA) and the top PI(3 P dots were quantified by Image J software.
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The areas enclosed by the dots were quantified (software Cell-P).
Fluorescent dots were quantified using ImageJ software (NIH).
Vector genomes (vg) were quantified by dot blot.
The dot intensities of apoptosis membrane arrays for semi-quantitative analysis were quantified by volume densitometry using BioRad Image Lab software.
Lung melanoma metastases were quantified by counting the number of colonies (which appear as black dots) on the pleural surface.
Results from these PLA experiments were quantified by counting at least 400 cells and expressed as the mean number of dots per cell as described in the Material and Methods Section (see Supporting Information Table S1).
Following the initial gate selection, the frequencies of cytokine+ cells were quantified by quadrant statistics applied on FSC/laser forward-scatter versus FL2/anti-cytokine-PE dot plots for monocytes and neutrophils and FL3/anti-CD16, CD8, CD8 or CD19 TC versus FL2/anti-cytokine-PE dot plots for NK-cells, T-lymphocyte subsets and B-cells.
The lipids were quantified by liquid scintillation.
Relevant bands were quantified by scanning densitometry.
The resultant bands were quantified by densitometry.
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