The binding energy of CdS quantum dots was analyzed by X-ray photoelectron spectroscopy (XPS; Sigma Probe, Thermo VG Scientific, Logan, UT, USA) using Al Kα radiation (1,486.6 eV).
The data were analyzed by drawing equal sized circles around each dot using ImageQuant software and obtaining a volume/intensity report for each circle.
Dot plots and histograms were analyzed by the Dako CyAn - Summit 4.3 software.
Dot plots and histograms were analyzed by CellQuest Pro software (BD Biosciences, Heidelberg, Germany).
The mixture and the clones of each aptamer obtained were analyzed by dot blot assay to confirm the target recognition, using the recombinant GST-amino terminal of PTEN protein or the synthetic peptide.
Subsequently the eluates were analyzed by dot blot with anti-hEPO antibodies and the positive fractions were pooled.
Mammaglobin A (SCGB2A2) and lipophilin B (SCGB1D2) expression were analyzed by dot blot analysis using Clontech's "Matched Tumor/Normal Array" (MTNA) and "Cancer Profiling Array I" (CPA) for each gene.
Resistant colonies, isolated from plates in which less than 30 wells contained resistant colonies to maximize the odds of isolating a true clone, were then transferred to 24-well plates in the presence of hygromycin B. Supernatant from confluent 24-well plates were analyzed by dot blot for the presence of IGF-E5.
Sequences corresponding to hits were analyzed by dot plot (http://www.vivo.colostate.edu/molkit/dnadot/), using standard parameters (window size 9, mismatch limit 0), or more relaxed conditions (window size 11, mismatch limit 1), to determine the exact start and end site of specific TCAST2 element.
Sequences corresponding to hits, as well as their flanking regions, were analyzed by dot plot (http://www.vivo.colostate.edu/molkit/dnadot/), using standard parameters (window size 9, mismatch limit 0), or more relaxed conditions (window size 11, mismatch limit 1), to determine the exact start and end site of specific TCAST-like elements.
