Adult Fisher and SD female rats were dosed with either a solution of PbAc containing 12 mg of Pb/ml or sodium acetate (NaAc) for controls.
For the assay, yeast cells were dosed with a serial dilution of E2β stock solution alongside a serial dilution of E1, E2α, E3, or lagoon extract stock solution; details are in Supporting Information.
For the assay, cells were dosed with a serial dilution of E2β stock solution alongside a serial dilution of E1, E2α, E3, or lagoon extract stock solution in RPMI 1640 media (Sigma Aldrich, St . Louis MO; 5% dextran-coated charcoal-treated fetal bovine serum vol/vol); details are in Supporting Information.
The females were dosed with an MeHg chloride (CH3HgCl) drinking solution 30 days before breeding through GD18.
The mixture was dosed with 0.2 molar solution of sodium trioxocarbonate IV, which on neutralizing the acid catalyst, stopped the reaction.
Specifically, a microneedle was used to inject the sciatic nerve with a single dose of a solution containing 1 µg of either sonicated M. leprae (n = 10) or cell membrane (n = 7) or LAM (n = 5) in a volume of 5 µl.
Moss visits with neuroscientists whose M.R.I.'s of test subjects demonstrate how the brain's so-called pleasure centers light up when the subjects are dosed with solutions of sugar or fat.
Because other safety steps may not prevent intravascular injection, test dosing with an epinephrine-containing solution may have value.
Brains were fixed 24 hours after the last BrdU dose by transcardial perfusion with a solution of 4% paraformaldehyde in PBS then post-fixed overnight at 4°C in the same solution.
17 Brown et al recently demonstrated that therapeutic levels of luliconazole were achieved across full-thickness human nail plate within 7 days of daily dosing with 10% luliconazole solution in an in vitro T. rubrum-infected nail model.
A typical "dose" is a solution so dilute it may not even contain a single molecule of the "cure".
