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We mixed dosing solution every other day and stored it at room temperature protected from light.
During dosing solution preparation, aerosolization of solutions needs to be avoided to prevent unintended exposure.
Dosing solution of 25 mg/mL was prepared by dissolving TMPP powder in physiological saline for the i.n. and i.v. administration, and dosing solution of 5 mg/mL was prepared by dissolving TMPP powder in distilled water for i.g. route.
Embryos were in dosing solution for 7 days, during which resorufin, the fluorescent product of CYP1A metabolism of ethoxyresorufin, accumulated in the embryos' bi-lobed urinary bladders.
A stock solution of A-1155463.7 (12.5 mg/ml) in DMSO was diluted by addition of 30% final volume of dosing solution Cremophor ELP ethanol (ratio 2 : 1) (Cremophor ELP, Sigma-Aldrich) and 5% dextrose in H2O (to reach final volume).
Using this technique, virtually 100% of the dosing solution is delivered directly to the lung.
Similar(30)
Dosing solutions of GLPG0492 and TP were prepared as solutions in a vehicle of 5% Ethanol and 95% Corn oil.
Dosing solutions of anti-mCTLA-4 mAb and hamster IgG control were prepared in sterile phosphate-buffered saline (pH 7.0).
Concentrations of PFOA in dosing solutions did not change appreciably over a 2-week period (data not shown).
Dosing solutions of PFOA dissolved in deionized water were analyzed from animal-watering apparatus to ensure no appreciable losses of PFOA occurred between changing solutions.
Aliquots of dosing solutions should be analysed for drug content by HPLC-UV, gas chromatography-mass spectrometry, liquid chromatography-mass spectrometry or other sensitive analytical methods.
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