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Sub-threshold doses of living syngeneic tumour, large doses of living allogeneic tumour and large doses of killed syngeneic tumour were without protective effect.
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Another group was given two intranasal doses of live attenuated influenza virus (LAIV).
In the case of whipworms this means patients swallowing doses of live eggs; in the case of hookworms they apply gauzes containing live larvae to their skin.
Three doses of live-attenuated OPV produce protective antibody to all three poliovirus types in more than 95% of recipients.
In fact, only 3 million doses of live attenuated vaccine were available at 5 months.
Finally, the long-term effect of repeated intranasal administration of high doses of live virus vaccines on the olfactory system is largely unknown.
Eligible subjects were randomly assigned using an interactive voice response system in a 4∶1 ratio to receive 2 doses of live monovalent H1N1 LAIV or placebo by intranasal spray 28 days apart (i.e., on days 1 and 29).
This pattern is similar to what we observed in larvae that were infected with high doses of live wild-type conidia (Figue 1 with 1×107 conidia per larva) or in the larvae whose immunity was pre-activated (Figure 10A).
A single dose of 106 pfu of live attenuated vaccine virus was weakly immunogenic and could protect mice from lethality despite high challenge virus replication in the respiratory tract, whereas two doses of live vaccine were required to protect mice and ferrets from pulmonary replication of challenge H5N1 viruses [17].
Two, MHC class I deficient mice (beta-2 microglobulin knock out) are not protected against heterotypic challenge and finally perforin deficient mice are not protected when gamma-flu immunized mice are challenged with lethal doses of live heterotypic viruses (Furuya et al, manuscript in preparation).
This process should translate into lower doses of live virus vaccine required to induce a robust and protective immune response.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com