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Effect of fish size and cercariae dose were tested in a 3 × 4 factorial design with 5 fish per combination of treatments (n = 60).
In each plate, one ZD1839 concentration and one radiation dose were tested.
Pathogen genotype effect was tested using the whole-plot error term, while the effects of host genotype, and inoculum dose were tested using the split-plot error term.
The effects of pathogen genotype and of block (inoculation day) were tested using the whole-plot error term, while the effects of host genotype and of inoculum dose were tested using the split-plot error term.
Further antiviral activities of the most active fractions at the highest dose were tested at days 1, 3, and 5. Analysis of inhibition of HBsAg and HBeAg secretions in the culture supernatants was done as mentioned above.
Several potential impacting factors for immunogenicity development, including naïve T-cell number, naïve B-cell number, binding affinity of T-epitopes to MHC-II, antigen dose, were tested for their impact on ADA generation by changing the parameter values over physiologically plausible range.
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Furthermore, the effect of a second ALA dose was tested.
One dose was tested per day under double-blind conditions and sessions were separated by at least 48 h.
This was not the case for 131I or 211At exposure at 24 h, where a broader range of absorbed dose was tested.
Each cocaine dose was tested twice under each medication condition, and the order of medication condition and cocaine dose were varied systematically.
Each cocaine dose was tested twice under each medication condition, and the order of medication condition and cocaine dose varied systematically.
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