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Therefore, any doses during the first 4 wk that would have been proportioned as a second dose were added as a first dose.
Fresh medium containing ethanol at 101.4 μL of 100% ethanol/100 mL of medium (80 mg/100 dL), FIV at MOI of 0.5, or ethanol (same dose) plus FIV (same dose) were added to these cultures for 24 hours.
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Between individual applications, the soil was dried in a vacuum oven to reduce the water content before the next dose was added.
In each sample, 3 g/l TCAC dose was added, and these samples were placed and agitated for 24 h in water bath shaker for different temperature in the varying range of 30 60 °C.
A maximal biofilm reduction was reached 8 h after each phage dose was added (e.g. 96% for initially 106 pfu added) (Figure 2A).
For bruceantin treatment, cells were rinsed with 1× PBS and fresh media containing bruceantin at the indicated dose was added for another 3 h.
No untoward effects or additional protection were noted if a third NTHi lysate dose was added on day 3. Observations through day 21 showed no subsequent mortality.
A second MMR dose was added in 1996.
200 μM 2,2'-dipyridyl as the optimal dose was added into LB medium for induction.
In 1996, to provide additional protection against all 3 infections, a second dose was added to the schedule.
GIK infusion: 10 mmol potassium-chloride and a calculated insulin dose are added to a 500 ml glucose 5% solution.
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