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Between individual applications, the soil was dried in a vacuum oven to reduce the water content before the next dose was added.
In each sample, 3 g/l TCAC dose was added, and these samples were placed and agitated for 24 h in water bath shaker for different temperature in the varying range of 30 60 °C.
A maximal biofilm reduction was reached 8 h after each phage dose was added (e.g. 96% for initially 106 pfu added) (Figure 2A).
No untoward effects or additional protection were noted if a third NTHi lysate dose was added on day 3. Observations through day 21 showed no subsequent mortality.
For bruceantin treatment, cells were rinsed with 1× PBS and fresh media containing bruceantin at the indicated dose was added for another 3 h.
A second MMR dose was added in 1996.
Similar(41)
GIK infusion: 10 mmol potassium-chloride and a calculated insulin dose are added to a 500 ml glucose 5% solution.
Therefore, any doses during the first 4 wk that would have been proportioned as a second dose were added as a first dose.
Fresh medium containing ethanol at 101.4 μL of 100% ethanol/100 mL of medium (80 mg/100 dL), FIV at MOI of 0.5, or ethanol (same dose) plus FIV (same dose) were added to these cultures for 24 hours.
SCF at different doses was added to the well such that the final volume in each well was 2 ml.
To trigger aggregation, ctDNA, λDNA or pBR322 DNA at given doses was added into the mixtures.
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