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Dose solution delivery rates were confirmed daily for each tank by collecting the outflow of dose solution over a timed interval and measuring the volume.
Twenty-five microfithes of the dose solution was pipetted into the trachea.
The flow rate, duration of dosing, dose solution concentration were optimized and adjusted according to the dosage required.
For the IN group, the anesthetized animals were held vertically, and 25 μL of the dose solution was pipetted dropwise to the naris until it was completely inhaled.
Test substance concentration or radioactivity of every dose solution was verified after preparation, before and directly after application to the skin samples.
The effect of the insecticide concentration, contact time, adsorbent dose, solution pH, temperature and inorganic ions on the biosorption was studied.
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Caffeine dose solutions were prepared in amphibian Ringer's solution, while testosterone was dissolved in ethanol/water 1/1 (v/v).
A volume of 10 µL/cm2 of the prepared dose solutions was applied topically to the skin, representing a finite dose of 40 µg/cm2.
Due to comparability, the dose solutions of each compound were prepared at a target concentration of 4 mg/mL since this is usually the applied concentration in reference studies on mammalian skin [28].
Preparation of dose solutions and estimation of mixture molar concentrations.
The dose solutions were prepared in 100% DMSO, aliquoted and stored frozen until used.
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