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Using this model for DHA cytotoxicity, we performed a dose response time course study (Figure S1) to identify a level of drug that resulted in significant cytotoxicity but not complete loss of the culture within 24 hours.
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To minimize the chance of off-target effects, we have performed experiments such as dose-response, time course and use of multiple controls including a 5-nucleotide mutated nestin MO.
Hormone dose-response time curves were estimated in three independent experiments performed with freshly isolated T. solium cysticerci.
10.7554/eLife.00508.006 Figure 4. Dose response and time course of selamectin's action in mESC cultures.
To investigate the involvement of NRF1 in iAs3+-induced antioxidant response in human keratinocytes, we measured the dose response and time course of iAs3+-induced NRF1 accumulation.
The dose response and time course of induction in the Panc02 cells were similar to the results seen in experiments on the MBT-2 cells.
Similar dose response and time course of induction of gene expression experiments were performed on the Panc02 cells containing the RHeoSwitch and the inducible reporter genes.
To determine the dose response and time course of selamectin action, we tested a wide range of selamectin concentrations (from 32 nM to 500 nM).
To reduce potential bias, eight candidate models were selected to characterize the bronchodilatory dose response over time and were prospectively specified.
In fact, considerable anti-inflammatory actions of GSNOR inhibition by N6022 were evident as noted by significant reductions in BALF eosinophils as well as BALF and systemic inflammatory biomarkers explored in both the dose response and time course OVA studies.
The optimal concentration of leucine, and duration of leucine uptake, was determined by dose response and time course experiments, showing 1 mM l-leucine for 30 min to be optimal (SI Figures S5C and D).
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