Various nitrogen compounds along with filtered SMW were tested as external dosing material.
An experimental methodology is presented that can provide means of assessment of the actual temperature of the dosed material.
For CENPs detected in the liver, the rare isotope ratio, 14C/26Al, was 87% of the dosing material's ratio.
This CENP was dosed to the rat and accelerator mass spectrometry (AMS) was used to quantify 26Al, 14C, and their ratio in the dosing material and tissue samples.
However, in brain and bone where little alumina was detected, the rare isotope ratio greatly exceeded that of the dosing material.
Because we did not detect monoPAPs in the feces after IV dosing, the concentrations observed after oral gavage presumably result from residual dosing material.
This resulted in exposures similar to that predicted on the basis of a single dose (Supplementary material).
This has the net effect of presenting the animal with a large bolus dose of material that, as discussed above, may overwhelm detoxification pathways.
Iodine flux (flow rate × iodine concentration) and iodine dose (contrast material volume × iodine concentration) are considered to be the determining factors for enhancement [ 4, 6].
Morpholinos were tested by injecting varying amounts into freshly fertilized zebrafish eggs and determining the maximum sublethal dose (supplementary material Table S3).
