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One day after the last dose, animals received an intracerebroventricular (i.c.v).
Elevations in epidermal cysts in high dose males and females in the intubation study and renal hyperplasia in high dose animals of both sexes in both studies may have a treatment relationship.
At the end of the study, bone loss occurred in the femur as indicated by decreased bone mineral density (BMD) in both LPS-treated groups, but vertebral BMD was reduced in the High dose animals only.
Dose injection of the repeat dose group was initiated 4 days prior to dosing of the single dose animals so that necropsies occur on the same calendar day for all groups, thus allowing for sharing of control clinical pathology and necropsy data between the two dose regimens.
Using NOAELs related to lowest LOAELs instead of lowest NOAELs, excluding studies with a dose spacing >8, and standardizing NOAELs to the initial dose animals received shifted the 5th percentile of NOAEL distributions from 0.22 to 0.5 mg/kg body weight per day.
Four hours after the last dose, animals were euthanized and tumors were resected, fixed with 4% paraformaldehyde and paraffin-embedded for immunohistochemistry staining and TUNEL assay.
Similar(40)
The reason for the difference in results is not clear but could be related to our lower selenium dose, animal model, and/or method of gene expression measurement.
The defined daily dose animal (DDDA) is the unit of measurement for the determination of annual veterinary antimicrobial drug consumption.
The radioactivity uptake was expressed as PSL/mm normalized for the injected radioactivity dose, animal weight and the radioactivity decay.
However, stratified analysis detected no significant influence of study quality, statin dose, animal species, drug delivery route, or anesthetic agent.
Hepatocellular vacuolation typical of fat accumulation was noted for both control and high-dose animals.
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CEO of Professional Science Editing for Scientists @ prosciediting.com