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In these cases, a trough is first taken around the end of an interval, followed by the administration of the next dose and collection of the peak sample.
Additionally, a common practice for sampling at steady state for drugs administered through III is to obtain the peak and trough samples from two subsequent dosage intervals: a trough sample is taken first, followed by the infusion of the next dose and collection of the peak sample [ 3].
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GFR was measured by inulin clearance (Inutest, Fresenius Kabi, Graz, Austria) using a continuous infusion of inulin after a loading dose and urine collections.
However, since it is difficult to obtain a concentration immediately after a drug has been administered (particularly if the same venous line must be used for both dosing and sample collection), it may be appropriate to fix the first sample collection at 1 hour.
The experimental procedures for sample preparation, TMA dosing, and data collection have been described in detail in our previous publication.
Because no investigational agents were administered and chemotherapy was given per standard dose and schedule, data collection was limited and safety reporting was not required for this phase of the trial.
Data was collected on film at 50,000× magnification and under low dose data collection conditions.
Collection was started at initiation of the loading dose, and all urine was collected in 6, 8, or 12 h aliquots, related to the PMA-dependent dosing interval.
Urine portions were collected during the first cycle on day 1, before the first dose, and post-dose with timed collections between 0 8 and 8 24 h after infusion.
Following peptide dosing and peripheral plasma collection, described above, plasma proteins were precipitated by addition of acetonitrile.
We also did not have an accurate data collection regarding the exact dose and duration of medications used by our diabetic patients, so we could not adjust for the dose and duration.
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