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Guinea pigs infected by low dose aerosol with the H37Rv strain of Mycobacterium tuberculosis rapidly developed granulomatous lesions in the pulmonary parenchyma and within the intra-thoracic hilar lymph node cluster.
LVS also protected against low-dose aerosol challenge (10 100 CFUs) with type A F. tularensis; however, it was unable to protect against a high dose aerosol challenge, the most likely dispersion route during a biological attack [2], [8].
All M. tuberculosis infections were performed by low dose aerosol infection (using an initial inoculum of 30 100 CFU in the lungs of the animals).
In the present study, similarly immunized mice were challenged along with naïve mice six weeks later with a low dose aerosol of SCHU S4.
From one month after low dose aerosol infection, the bacilli were mainly extracellular within the necrotic core and surrounding acellular rim of the primary granulomas.
Treatment was initiated 18 days after low dose aerosol infection (standard protocol) and lasted up to 28 days after aerosol infection.
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Usually, the metered-dose aerosol or inhaler is placed in the mouth for use.
Meanwhile, Sciele Pharma, based in Atlanta, plans to seek approval from the agency next year to market a prescription drug in the form of a metered-dose aerosol sprayed on the skin that is intended to increase latency time.
To model these aspects the guinea pig low-dose aerosol infection model is developed because it shares these clinical features with the human disease.
A high intersubject variability of lung deposition was observed with both modes regarding lung doses, aerosol penetration and distribution between the right and the left lung.
The animals were then infected by a low-dose aerosol of M. tuberculosis (M.tb).
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