For dorsal cord imaging, young adult worms, in which the dorsal cords were oriented toward the objective, were imaged in the region midway between the posterior gonad bend at the tail.
Thus, the aim of the present work was to test, in typical patients with ALS with distal motor dysfunctions in upper limbs and no sensory symptoms, whether abnormal metrics of dorsal column imaging are associated with altered SEPs, with a focus on N9 and N20 components, which depend mainly on the peripheral afferent volley.
We have previously demonstrated SV in a low BTM scenario by imaging mouse dorsal skinfold window chamber, where small capillaries could be detected with relatively slow image speed of 36kHz by optimizing the SV parameters (i.e. gate length, n, or the number of frames used to calculate the SV, and frame rate, F) [ 6].
If we posit that the larva detects molecules within 1 mm around the dorsal organ in the behavior assay and within 1 mm around the dorsal organ in the calcium imaging experiments, then the concentrations used 50 fm/cm and 1 nM translate to 3.0x10 molecules and 6.0 x10 molecules, respectively.
For experiments that used larvae 4 dpf or beyond, the skin covering the brain was removed using sharpened tungsten needles (under modified ACSF containing 0.02% MS 222) to expose the brain for imaging from the dorsal side.
Hendrickson et al. (2007) also reported imaging sacculations in both dorsal and ventral sites, and attributed this to peristaltic activity causing segmentation of non-sacculated dorsal colon.
Brains were imaged horizontally from the dorsal side, and then rotated 180° for imaging from the ventral side.
The large dorsal vein and extensive tributaries prevented imaging microvascular networks beyond 200μm.
Medial-lateral (ML) and dorsal-ventral (DV) spread was measured using imaging program, IMAGEJ (National Institutes of Health, USA).
At the end of the training, odorants evoked input patterns are monitored on the dorsal OB of awake mice using intrinsic optical signal imaging.
