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The liposomal formulations were cationic in nature, and consisted of PC, dioleoylphosphatidylethanolamin (DOPE), 1,2-dioleoyloxy-3-trimethylammoniumpropane (DOTAP) (4∶2∶1 molar ratio) prepared by the addition of materials to small unilemallar vesicles (SUVs) and processed by the DRV method [20].
1,2-dioleoyl-sn-glycero-3-phospho-L-serine (DOPS), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), and cholesterol (supplied in chloroform from Avanti Polar Lipids) were mixed in molar ratios of 3 3 2 2, respectively.
1,2-dioleoyl sn-glycero-3-phosphoethanolamine (DOPE), 1,2-distearoyl sn-glycero-3-phosphoethanolamine- N-[carboxy polyethyleneglycol 2000], ammoN-[carboxy polyethyleneglycol 2000]orescein-5-thiocammonium)-1,2-dihexa-decanoyl- saltlycero-3-phosphoethanolamine, triethylammonium sand (FL-DHPE) were purchased from AvaN- fluorescein-5-thiocarbamoyl -1,2-dihexa-decanoyl-
The lipids DOPC (1,2-dioleoyl sn-glycero-3-phosphocholine), DOPE (1,2-dioleoyl sn-glycero-3-phosphoethanolamine), DOPS (1,2-dioleoyl sn-glycero-3-phospho L-serine) and POPC (1palmitoyl-2-oleoyl sn-glycero-3-phosphocholine) were purchased from Sigma, resuspended in chloroform to create 2 mM stocks and PtdIns 3,4,5) P3 was resuspended in chloroform/methanol/water (5:10:4, by vol).
This was achieved by mixing 60 mol% DOPC, 25 mol% DOPE, 15 mol% DOPG from stock solutions.
1,2-Dioleoyl sn-glycero-3-phosphoethanolamine (DOPE: 18 1 (Δ9-Cis) was purchased from Avanti Polar Lipids (Alabaster, AL).
A photo posted by 2 Dope Queens (@2dopequeens) on Mar 24, 2016 at 11 20am PDT.
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