Since fibrillar type II collagen was previously shown to induce IL-6 release from chondrocytes [ 32, 33], we also measured the release of IL-6 from P2 chondrocytes (n = 3 healthy donors) seeded at a density of 1.6 × 106 cells per cm2 on filters coated with type I collagen (rat tail; BD Biosciences, #354249), and denatured type I and II collagen.
Therefore, the tissue culture system (donor seed-plants, calli and regenerants) of these rice ssp.
These three types of bands should correspond, respectively, to static, excised and newly inserted mPing copies in the calli and/or regenerant(s) relative to their donor seed-plants in a given genotype.
Bands showing various patterns of genetic or methylation alterations in calli and/or regenerated plants (regenerants) relative to the donor seed-plants were eluted from the silver-stained AFLP or MSAP gels and re-amplified with the appropriate selective primer combinations.
In addition, by taking advantage of the draft genome sequence of the indica rice cultivar 93 11 [ 38], locus-specific primers were designed for each of the loci, and the corresponding putative " mPing-empty loci" were also amplified from the donor seed-plant, and sequenced (Additional file 2).
Consistent with prior research, informing donors that seed money has already been raised increases donations, as does a $1 $1 matching campaign.
We supplement the measures of relatedness between mating pairs with estimates of the number of pollen donors per seed crop (Nep) and multilocus outcrossing estimates using the program MLTR v 2.4 [88].
Corneal epithelial cells freshly isolated from 3 separate donors were seeded directly onto the peptide-conjugated contact lenses and compared with cells seeded onto glass coverslips.
Working viral stocks were prepared for this study by infection of CD8-depleted PHA-stimulated PBMCs from a single donor using seed stocks maintained in the uQuant Core Facility at the Institute of Human Virology.
hMSCs from 9 donors were seeded at 1,000 cells/cm.
