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Moreover, a second sample from the same donor was collected by a 5-fold acceleration of blood flow.
The flow through during the CD14+ selection of PBMC (same donor) was collected, and the purity of the lymphocytes (PBL) was tested by flow cytometry using CD14 and CD3 antibody.
Venous peripheral blood (60 ml) from a healthy donor was collected in a lithium-heparin BD Vacutainer tube (BD, Franklin Lakes, NJ, USA).
Blood (8 ml) from each donor was collected in serum separator tubes (Becton Dickinson) and centrifuged (2000 g, 10 min) at room temperature.
Blood from each healthy donor was collected into EDTA tubes (BD Biosciences, San Jose, CA, USA) or CellSave tubes (Veridex, LLC, Raritan, NJ, USA).
27 mL of blood from each donor was collected into Sodium Citrate tubes (Becton Dickinson, New Jersey, catalog# 366415) and mixed well.
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Hematopoietic stem cells from a bone marrow donor are collected using apheresis.
Approximately 2 ml of blood samples from each donor were collected into four pools, according to different blood groups (blood group A, B, O and AB).
Blood from 116 blood donors was collected with written informed consent in serum glass.
Peripheral blood from two healthy, HLA-mismatched donors was collected via apheresis.
Whole blood of volunteer donors was collected into a BD vacutainer (Becton Dickinson, NJ, USA).
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