Sentence examples for donor templates from inspiring English sources

The donor templates are marked by BamHI and EcoRI sites, which are absent in the parental cassettes.

These results suggest that co-injected single-stranded oligodeoxynucleotides (ssODNs) as donor templates preferentially support the activation of HDR relative to the non-homologous end-joining pathway.

Due to the presence of Flex1 and Luc in the donor templates, HR would not produce green cells and PCR analysis was used to score the repair events.

We also tested an IDLV construct for delivery of donor templates and demonstrated its efficacy for gene modification.

The Cas9 endonuclease, along with an sgRNA, introduces sequence specific DNA double-strand breaks which results in non-homologous end joining (NHEJ -mediated iNHEJ -mediateddeletinsertionsmology-directed repand (Hdeletionsted repair in the presence or donor templates.

To test this hypothesis, we designed DSB repair substrates (HR-Flex/D-Flex and HR-Luc/D-Luc, Fig. 5f, top) with Flex1 or Luc also inserted in the EGFP donor templates.

The 173-bp ssODN donor template is presented under the genomic DNA with a R229Q mutant sequence in green.

The transfer product appeared with much slower kinetics than full-length synthesis on the donor template.

Sather BD, Romano Ibarra GS, Sommer K, Curinga G, Hale M, Khan IF, et al. Efficient modification of CCR5 in primary human hematopoietic cells using a megaTAL nuclease and AAV donor template.

To convert the BV to a gene targeting vector, a DNA donor template and ZFNs were incorporated into the vector.

"We have developed a strategy that physically links the blueprints for sgRNA and donor template in one stable and heritable extra-chromosomal DNA molecule (guide+donor).