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The synchronization of the donor stimulation with the endometrial preparation of the recipient is usually done by downregulating the recipient's pituitary with a GnRH analog.
To compare pregnancy rates and the incidence of ovarian hyperstimulation syndrome (OHSS) in donor stimulation cycles where final maturation of oocytes was induced with recombinant hCG or GnRH agonist.
The large sample size of the current study, together with the standardization of the donor stimulation protocols, the recipient endometrial preparation and the sperm sample evaluation provides enough scientific weight to conclude that paternal age does not play a significant role in determining the results of ICSI when the oocyte is young and healthy.
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Eight recipients had the above defined ratio of <1, with significantly decreased interferon-γ secretion after donor versus non-donor stimulation.
Within the CD4 population, two distinct patterns of CD69 and CD71 expressions were observed: recipients who had a lower percentage of CD4CD69 and CD4CD71 cells after donor versus non-donor stimulation (therefore a donor/non-donor ratio <1); and recipients who had a higher percentage of CD4CD69 and CD4CD71 cells after donor versus non-donor stimulation (therefore a donor/non-donor ratio ≥1).
In CD4+ T cells of healthy donors, stimulation by anti-CD3/anti-CD28, caused a transient increase of the TRPM2 expression [ 124].
An in vitro study also revealed that treatment with anti-OX40L monoclonal antibody reduced the proliferative response and interferon-gamma production of lymph node cells after donor alloantigen stimulation.
However, they did not respond to donor alloantigen stimulation.
Next we investigated the response of blood lymphocytes from the ten healthy adult donors to stimulation with live S. Typhimurium D23580 for six hours (Figure 4A).
In contrast to intracellular IFNγ analyses, some IFNγ was also detected in supernatants of PBMC from a subgroup of TST-negative donors after stimulation with latency-associated antigens as described previously [5].
In contrast to intracellular staining, a considerable amount of IFNγ was also detected in supernatants of PBMC from some TST-negative donors after stimulation with latency-associated antigens (figure 2B).
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