Therefore, the tissue culture system (donor seed-plants, calli and regenerants) of these rice ssp.
These three types of bands should correspond, respectively, to static, excised and newly inserted mPing copies in the calli and/or regenerant(s) relative to their donor seed-plants in a given genotype.
In addition, by taking advantage of the draft genome sequence of the indica rice cultivar 93 11 [ 38], locus-specific primers were designed for each of the loci, and the corresponding putative " mPing-empty loci" were also amplified from the donor seed-plant, and sequenced (Additional file 2).
Bands showing various patterns of genetic or methylation alterations in calli and/or regenerated plants (regenerants) relative to the donor seed-plants were eluted from the silver-stained AFLP or MSAP gels and re-amplified with the appropriate selective primer combinations.
However, in campaigns targeted at high fidelity donors, seed money is an effective strategy only when the threshold is rather high.
Since fibrillar type II collagen was previously shown to induce IL-6 release from chondrocytes [ 32, 33], we also measured the release of IL-6 from P2 chondrocytes (n = 3 healthy donors) seeded at a density of 1.6 × 106 cells per cm2 on filters coated with type I collagen (rat tail; BD Biosciences, #354249), and denatured type I and II collagen.
Working viral stocks were prepared for this study by infection of CD8-depleted PHA-stimulated PBMCs from a single donor using seed stocks maintained in the uQuant Core Facility at the Institute of Human Virology.
Consistent with prior research, informing donors that seed money has already been raised increases donations, as does a $1 $1 matching campaign.
On June 2008, the first human tissue-engineered trachea replacement was performed using decellularized (de-antigenised) cadaveric donor trachea, seeded with recipient epithelial cells on the internal surface of the graft and mesenchymal stem-cell-derived chondrocytes on the external.
Cryopreserved human hepatocytes from a single donor were seeded within the HμREL® device to predict the in vivo hepatic clearance (CLH) of six marketed model compounds (carbamazepine, caffeine, timolol, sildenafil, imipramine, and buspirone).
We supplement the measures of relatedness between mating pairs with estimates of the number of pollen donors per seed crop (Nep) and multilocus outcrossing estimates using the program MLTR v 2.4 [88].
