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Additionally twelve healthy donor samples were used in RT-PCR experiments.
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The OA group included 7 synovial fluids of the same donors from whom cartilage samples were used for TN-C protein and mRNA expression.
Total RNA from each individual donor and sample was used for genome-wide gene expression profiling.
In general, each donor's BM sample was used for running >15 coculture experiments.
Genes that were more than 2-fold up- or down- regulated in median of all three donor samples were further classified by GeneOntology analysis using GoMiner software (http://discover.nci.nih.gov/gominer/) and representation in functional categories was analyzed by Fischer's Exact p-value test (P<0.0001).
Seventy one donor samples were not suitable.
The donor samples were anonymous and deidentifed.
DNA from blood of some patients and from blood donor samples was also extracted using the same procedure.
Fifty peripheral blood samples from healthy donors were used as the training set for the establishment of MLPA analytical and interpretative parameters.
Peripheral blood samples from healthy donors were used as controls.
Twenty-four blood samples from healthy donors were used as controls.
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