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The changes of carrier concentration and other factors affect the dye concentration, donor, membrane and acceptor phases.
Vesicles budded from the donor membrane, moved to the receptor, anchored in the membrane receptor, and fused with membrane receptors.
where Cdo is the initial dye concentration in the donor phase, while Cd, Cm and Ca represent the dye concentrations in donor, membrane and acceptor phases, respectively.
where Cd0 is the initial mercury II) ion concentration in the donor phase, while Cd, Cm and Ca represents the mercury II) ion concentration in donor, membrane and acceptor phases, respectively.
The trafficking of proteins within eukaryotic cells is achieved by the capture of cargo and targeting molecules into vesicles that bud from a donor membrane and deliver their contents to a receiving compartment.
It has been noted previously that in addition to providing attachment sites for motors [56] or directly moving vesicles from the donor membrane through polymerization [57], [58], actin might also provide a barrier to vesicle formation [59].
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Myosin might also act in cargo protein assembly at donor membranes, as recently suggested for the biogenesis of melanosomes [50], [51].
The latter cycle is mediated by the Guanine Nucleotide Dissociation Inhibitor GDI, which can selectively extract GDP-bound Rab proteins from donor membranes, and then reload them on target membranes.
Lysates of purified vesicles or donor membranes were probed with the indicated antibodies.
The coat proteins cause trafficking vesicles to bud from donor membranes and to traffic to acceptor membranes.
The small GTPase ARF1 promotes vesicle budding from various donor membranes, including Golgi membranes (Serafini et al., 1991a; Bremser et al., 1999).
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