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For transplantations between individuals, one dorsal scale from the donor fish was inserted into the ventral region of the recipient fish.
Briefly, RCPT fish received 1 ml of intestinal scraping homogenates in Hank's Balanced Salt Solution (HBSS) from 20 donor fish, containing E. scophthalmi live parasites, whereas CTRL fish were inoculated with the same amount of HBSS alone.
This was achieved by anaesthetising the individual with 0.02 % MS222 and bringing it into contact with a heavily infected donor fish until four worms had transferred from donor to recipient (taking <5 min).
Without one further generation of inbreeding only 3 in 256 donor fish will have at least one copy of the transgene and be homozygous for the three mutations; therefore we did not carry out these experiments.
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Choline is involved in various physiological functions such as neurotransmission, betaine synthesis and transportation of lipid molecules, and it acts as a methyl donor in fish tissues (Halver 1989; Mai et al. 2006).
Infection was achieved by anaesthetising each focal individual and allowing them to contact a euthanized heavily infected same-sex fish (donor) in a watch glass containing 0.02% MS222 on the stage of a stereo-microscope.
But I'm an incurable optimist, so I want "big fish" donors too since all proceeds over $3,000 will be given equally to The Corporation for Supportive Housing (CSH), and Feeding America (formerly America's Harvest).
Foreign donors built the fishing port here as well as a large cargo airport, a container port, extensive roads and a modern phone system, hiring security guards from rebel forces and buying sand, gravel and other construction materials from rebel leaders' businesses.
For experiments to investigate the development of injected fresh and frozen/thawed spermatogonia and generation of spermatozoa after transplantation, wild-type coloration adult Nile tilapia (Chitralada strain) were used as donors and recipients fishes.
Gut microbiota transplantation with cecal content from donor mice fed lard or fish oil for 11 weeks was performed on male 12-week-old mice.
Courting major donors is like catching big fish on light tackle: pull too hard and the line breaks.
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