Sentence examples for donor cells using the from inspiring English sources

DNA was extracted from the cloned blastocysts and from the donor cells using the kit Genomix, according with the manufacturer instruction.

RNA was isolated from IVF blastocysts, SCCT blastocysts, and donor cells using the RNeasy MicroKit (Qiagen Valencia, CA) according to the manufacturer's specifications.

The analysis clearly showed that the gene expression profiles of the SECNT embryos (71 samples) were invariably clustered into 3 groups corresponding to the type of donor cell used: the CUCB, SRCB, and ESCB groups.

Therefore, several studies have attempted to modify the epigenetic status of donor cells using variants of HDAC inhibitors either before or soon after SCNT.

If the tracking of donor cells in the recipient is necessary, it is possible to use flow cytometry to track MHC disparity, to track fluorescent donor cells using BLI, or to use congenic mice that have allelic differences at the CD45 locus (e.g. CD45.1 or CD45.2) to track cellular subsets from donor versus recipient when they have a common MHC.

In turn, the ability to shorten the time required for efficient in vitro expansion of donor cells using smaller amounts of testicular tissue could prove to be critical for cancer survivors that bank their spermatogonial stem cells prior to therapy.

By gating on donor cells using a congenic marker and assessing dual expression of foxp3 and GFP, we found both decreased Treg infiltration and loss of foxp3 expression within the tumors of DTA-1-treated recipients (Figure 7A), implying that both of these mechanisms are playing a role in this phenomenon.

To determine gap junction activity, recorded images were analyzed for the density of calcein dye uptake by unlabeled cells and calcein retained by DiD-labeled donor cells using ImageJ.

To confirm that the puromycin-resistant GFP expressing cells were generated through fusion, we first assayed the genetic markers carried by the donor cells used for the fusion.

A key difference, however, is that Dolly's donor cell came from adult udder cells growing in lab dishes (see ScienceNOW, 24 February), while the donor cells used to create the monkey clones came from early embryos.

However, failure to execute differentiation in a small fraction of cells that could contaminate the donor cells used for transplantation is also critical to PSC tumorigenicity, as the most common tumor type documented after transplantation of differentiated donor cells derived from PSCs are teratomas [3], [12], [13], [14], [15], [16].