Cells expressing this mCherry-CadC hybrid responded to external stimuli like the wild type did (Supplementary Figure 1).
We also found that apoptosis of CD4+ iTSCM cells after TCR restimulation was significantly lower than CD4+ iTCM cells did (Supplementary Fig. 10b).
As a result, whereas all the clones cocultured with OP9-DL1 cells produced CD44loCD62Lhi iTSCM cells, none of the clones with OP9 cells did (Supplementary Fig. 3).
OVA-specific CD8+ iTSCM cells also proliferated faster in E.G7-OVA-bearing mice in response to OVA restimulation than OVA-specific iTCM cells did (Supplementary Fig. 12a).
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CD8+ iTSCM cells also composed a higher proportion of G2/M and S phases and fewer AnnexinV+PI+ apoptotic cells than CD8+ iTCM cells did (Supplementary Fig. 11a,b), which is related to a lower expression of p53 and its target genes than in CD8+ iTCM cells (Supplementary Fig. 11c,d).
Similarly, OVA-specific CD4+ iTSCM cells also survived in the sentinel lymph nodes and suppressed E.G7-OVA cell growth more efficiently than CD4+ TEM and TCM cells, iTCM cells, and CD62L+ cells, although CD4+ iTSCM cells showed weaker tumour suppressing effects than CD8+ iTSCM cells did (Supplementary Fig. 12b,c).
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On day one, students worked within their teams to answer a series of questions like "Who are the primary ocean interest groups in your country?" "What are your priorities for ocean governance?" and "What treaty design best serves your interests?" Students were instructed to work with their teammates, and to do supplementary in-class research to help flesh out their positions.
