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These manipulations are done in vitro with enzymes that cut and ligate DNA in new ways.
In case of non-availability of sequences, sequencing has to be done in vitro for which a recently developed software ecoPrimers can be helpful.
We've done in vitro fertilization.
This was originally done in vitro with vector constructs and cell-free extracts [28] and later in peripheral blood cells [29] but has not yet proceeded to clinical trials.
The binding of 18F-nifene to α4β2 receptors of the rodent brain in microPET studies gave results consistent with the receptor distribution and was comparable with the autoradiographic slices done in vitro [3].
Consequently, morphometric analysis, upon which implants are designed, are frequently based on measurements at the bone-cut levels done in vitro (Kwak et al. 2007), in vivo (Hitt et al. 2003; Westrich et al. 1995) or based on CT scans (Bonnin et al. 2016; Bonnin et al. 2011).
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Largely the assessment of bonding agents has been done in-vitro using shear bond strength measurement.
It is clearly not known without testing what the minimal effective dose would be and there will have to be both neutralisation tests done in-vitro and some dose escalation experiments to see what dose is effective in stopping early infection from taking hold.
"I did in-vitro and paid someone for her eggs!
We are currently doing in-vitro maturation and selection to confirm that they can potentially mature to VRC01-like bnAbs.
However, also for this method the requirement to do in-vitro refolding presents an important practical limitation.
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