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All the experiments were done in triplicate to ensure the reproducibility of the data.
All samples, including controls and standards, were done in triplicate to control for discrepancies in pipetting.
Each experiment was done in triplicate to confirm reproducibility of the results, and representative results are shown.
All experiments were done in triplicate to verify the results.
This process was done in triplicate to minimize PCR amplification biases.
Each simulation was done in triplicate to assess the consistency of the data.
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Hundred explants were used in each concentration and were done in triplicates to get the significant regeneration efficiency.
All the experiments were done in triplicates to ensure reproducibility.
The experiments were done in triplicate, normalized to 100% for untreated parasites.
Each PCR was done in triplicate and normalized to endogenous 18s gene using Taqman Fast Reagent Starter Kit ABII).
Each reaction was done in triplicate and normalized to endogenous 18 S gene using Taqman Fast Reagent Starter Kit ABII).
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