Sentence examples for done in the same buffer from inspiring English sources

Elution was done in the same buffer but in the presence of 250 mM imidazole.

Hybridization was done in the same buffer reducing skim milk to 0.25% and adding 10 μg poly-dI dC and 10 Cpoly-dI dColandled probe.

Electrophoresis was done in the same buffer at 25 V and 300 mA for 20 min to allow the damaged DNA or fragments to migrate towards the anode.

These processes can be done in the same buffers so that no additional transfers are needed.

FaPy DNA glycosylase assays were done in the same reaction buffer as above and [ N-H]methyl N′-nitrosourea (18 Ci/mmol) was used to prepare poly (dG·dC) DNA containing faPy residues (5000 d.p.m./μg DNA) as described (29).

Control experiments were done in the same way except that all buffer solutions contained 10 mM KCl.

Alternative pathway (AP50) assays were done in the same way, but with DGVB-Mg-EGTA as buffer.

Immunoprecipitations were done in the same way, with the exception of using 1% NP-40 lysis buffer.

Column was washed with 10 mM imidazole (Sigma) 0.3 M NaCl in sodium phosphate pH 8.0 and elution was done using 250 mM imidazole in the same buffer.

The two GFP fusion proteins were able to induce necrosis when infiltrated on leaves, while infiltration with GFP alone (Roche, http://www.roche-applied-science.com) dissolved in the same buffer, or with the buffer alone, did not show any effect.

Sera were similarly 4-fold diluted in the same buffer.